|
KMID : 0545119960060060386
|
|
Journal of Microbiology and Biotechnology
1996 Volume.6 No. 6 p.386 ~ p.390
|
|
|
Molecular Cloning of a β-D-Galactosidase Gene from Lactococcus lactis subsp. lactis 7962
|
|
CHANG, HAE-CHOON
CHOI, YANG-DO/LEE, HYONG-JOO
|
|
|
Abstract
|
|
|
|
The β-glucosidase gene from Lactococcuse lactis sunsp. lactis ATSS 7962 was cloned and its enzymatic properties were characterized, with a view to assessing its potential use as a selection marker in the food-grade cloning vector. Chromosomal DNA from L. lactis sunsp. lactis ATSS 7962 was cleaved with PstI and ligated into pBR322 for transformation into Esherichia coli TG1. Transformants showing β-glucosidase actuvuty possessed the pBR322 plasmid containing a 10 kilobase (kb) pstI fragment and this plasmid was named pCKL11. The cloned β-glucosidase gene came from the chromosomal DNA of L. lactis sunsp. lactis ATSS 7962 was confirmed by Southern hybridization. A restriction map of pCKL11 was constructed from the cleavage of both pCKL11 and the purified 10kb insert fragment. The optimal pH of the β-glucosidase determined with the E. coli harboring the pCKL11 was 7.0. The optimum temperature was 50℃, while the pI if the enzyme was 7.4. These values were the same as those of the enzyme from the parent strain.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|